ALPHA3
Alpha3 is a new generation of light sheet microscope addressing the needs of high temporal resolution along with spatial high-resolution to achieve qualitative and quantitative 3D imaging of fixed or live biological specimen.
From in-vivo imaging to large cleared samples, the Alpha3 microscope delivers unprecedented image quality while keeping the necessary flexibility and modularity expected for cutting-edge scientific research instruments.
Typical applications of Alpha3 include in toto imaging of small animal models such as whole mouse embryos, morphogenesis and embryogenesis of model organisms: C.elegans, Drosophila, Zebra fish, live imaging of cell cultures, functional imaging of neuronal activity, fluorescence imaging of marine organisms or plant developmental biology.
From in-vivo imaging to large cleared samples
Macro to micro view imaging, from whole organs at sub-cellular resolution to very small specimen; Compatible with all clearing solutions: aqueous buffers and organic solvents
From very small to large specimen
Versatile chamber with easy access; Multiple accessories provided for sample mounting to accommodate a large variety of specimens
Smart light sheet illuminators
Multi-directional light sheet performing real time laser focus sweeping to extend the thinnest focus area over the entire field of view, artefacts-free imaging and ultra fast Z-stacking
Modular light sheet microscope
Flexible imaging system with a large selection of detection objectives: 2X to 60X, air, dipping lenses, clearing objectives with correction collar for RI matching; Fully flexible for easy adaptation to any experiments; Open microscopy system for multimodal optical microscopy: FLIM, FRET, FRAP, photo ablation ..
Specification
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Light source |
Laser combiner with up to 4 laser lines: fibered lasers CW / Laser diodes or DPSS Wavelength selection from 405 nm to 785nm, output power from 25 to 250mW. |
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Light sheet |
Dual smart illuminators with fibered connection to laser combiner. Multi-directional light sheet with real time focus sweeping Chromatic correction 400 ‐ 632nm Minimum light sheet thickness 2µm, range: 2µm - 12µm, width: 2mm - 15mm |
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Chamber & Holders |
Chamber dimensions : width 21mm, length 70mm height 25mm; volume < 15ml Chamber highly resistant to various corrosive medium, clearing agents, sea water, Sample size from µm to cm range Multiple holders for sample mounting: molds, coverslips , glass supports Optional: temperature and CO2 controls. |
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Volume scanning |
Z motorized stage: range 15mm, precision 0.1µm, acquisition speed 40 fps Optional: XY motorized stages for tiling: range 15mm, precision 0.1µm Optional: ultra fast 3D scanning module (75 images /second) |
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Detection unit |
Fluorescence microscope stand comprising objective slider 2 positions , eyepieces, video port, motorized filter wheel, multi-notch filter |
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Detection lenses |
Large selection of long working distance objectives : air ,dipping lenses, clearing objectives with correction collar RI 1.33 - 1.56 Magnification: 2X to 60X |
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Image Sensor |
sCMOS 2048 x 2048 pixels, format 13 x 13 mm, 6.5µm square pixels., USB3 / CameraLink Interface |
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Software |
QtSPIM software for stacks, XY tiling and time lapse acquisition. Easy export of raw images and metadata to open source or commercial 3rd party software |
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PC |
Desktop i7-7700K 4.2Ghz – 2 x 16Go RAM - GPU GeForce GTX1060-6Go - Hard Disk 4 x 4To RAID0 - Hard Disk SSD 500Go , QHD Screen 31.5”, Windows 10 / 64b Pro |
Software
From sample mounting to image acquisition, Alpha3 performs seamless light sheet imaging. The QtSPIM software provides clear and intuitive interface to collect X,Y,Z,θ,T,λ images at maximum speed. QtSPIM controls all image acquisition parameters. Along with a workstation grade computer with an optimized configuration, the microscopy system performs ultra fast acquisition
Raw image data with their metadata are saved in 16 bits TIFF format, compatible with open source or commercial software for further 3D display and analysis
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