ROS Indicator

LumiSTAR provides various protein-based genetically encoded ROS indicators with viral delivery and expression systems. The products have superior properties as following:

  1. Easy operation & less toxicity
  2. Steady expression for long term recording (Up to 1 month)
  3. Real-time observation & dynamic functional test
  4. Observation on different organelles
  5. Customized design is available

 

  ROS Indicators

  

Reactive oxygen species (ROS) are produced as an inevitable byproduct of oxidative phosphorylation in healthy and cancer cells. Cancer has higher ROS from increased metabolic activity, mitochondrial dysfunction, increased cellular receptor signaling and other oncogene activity, but cancer can tolerate it by increase the expression of ROS scavenger. Here we generated genetically encoded H2O2 sensor that has high sensitivity for ROS, allows for the ratiometric real-time analysis of ROS generation in living cells. roGFPs sensor can be excited by blue light with wavelength 405 and 488nm and detected at 510nm. The ratio of 405nm/488nm indicates the redoxstatus within the cells.

 

 

 

 

 

 

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