SARS-CoV-2 (COVID-19) Pseudovirus for Neutralizing assays

LumiSTAR used the lentiviral backbone to package the SARS-CoV-2 (2019-nCoV, COVID-19 virus) S protein as a surface capsid glycoprotein and containing GFP and Luciferase dual reporter genes as a pseudovirus model. The virus can infect cell lines overexpressing the ACE2 gene. Researchers can determine the infection efficiency by observing GFP through a microscope and detecting the value of Luciferase.

Neutralization Assay- The inhibition of viral entry into cells by Neutralize Antibody is correlated to the decreased levels of GFP fluorescence or luciferase signals in the cells. This method is superior to the conventional assay because of its simplicity, higher sensitivity and accuracy, suitability for high-throughput experiments. In addition, no live virus is used during the test. Therefore, this method could be used as an alternative for safely conducting serologic studies in a rapid response in assessing the threat posed by SARS-CoV-2.

Example of Application