ORCA-Lightning Digital sCMOS camera, 高感度相機(高靈敏度相機) , 科研相機

第二代sCMOS相比,提供2倍的像素面積,2.8倍像素和3.4倍每秒像素讀數。

 

Effective no. of pixels
4608 (H)×2592 (V)
Readout speed
121 frames/s
Readout noise
2.0 electrons
Dynamic range
17 000 : 1


Special sites

The scientific camera section is the feature section for digital cameras fit to life science research and industry areas.

 

Special site for Scientific cameras 

Features

High resolution : 4608 x 2592 (12 Megapixels)

High Speed: 121 frames/s (at full resolution)

Read noise : 2.0 electrons rms (Standard Full Well Capacity mode)

Pixel size : 5.5 μm X 5.5 μm

Dynamic Range 17 000:1 (High Full Well Capacity mode)

Peak QE : 60 % (at 550 nm)

High-speed imaging

Can even take high-speed images which were difficult to fully capture until now, with approximately 6 times the readout speed, while maintaining a level of readout noise similar to that of ORCA-Flash4.0 V3.

 

C14120-20P Comparison of speed

Speed comparison with ORCA-Flash4.0 V3

 

Cameras used in light-sheet microscopes put emphasis on high speed, demanding fast readout. Because images with increased readout speed result in obtaining fewer photons upon capture, it is necessary to keep the camera's readout noise low when trying to capture high S/N images. ORCA-Lightning can capture images at approximately 6 times the readout speed, while maintaining a level of readout noise similar to that of ORCA-Flash4.0 V3.

Using CoaXPress as an interface and optimizing high speed data transfer to the PC, ORCA-Lightning realizes high-speed image acquisition.

 

Readout speed (for each effective number of pixels and modes)

Customers can select the best mode depending on their usage.
In addition to a readout from a Standard Full Well Capacity mode (12 bit), ORCA-Lightning also supports a readout from a High Full Well Capacity mode (16 bit) for high dynamic range.

*1 Free running mode is operated via camera alone.
*2 Edge trigger mode starts the exposure by the edge (rising/falling edge) of the trigger signal input to the external device.
*3 Synchronous readout trigger mode ends the exposure of the camera and readout is started, while the next exposure is simultaneously started by the edge (rising/falling edge) of the trigger signal input to the external device.
*4 Global reset trigger mode starts the exposure of all pixels all together by synchronizing the trigger signal from external device.

 

High speed test of 3D imaging

We took consecutive images and produced 3D imaging of a free-swimming Volvox.
When capturing the image of a free-swimming Volvox, imaging speed of 2-dimensional (area of observation) and 3-dimensional (number of images) settings were selected with a precondition of approximately 8 volumes per second.
With its high speed, ORCA-Lightning can capture clearer images within a wide range compared to ORCA-Flash4.0 V3.

Imaging conditions

  • Microscope: ezDSLM (light-sheet microscope)
  • Objective lens: 10×
  • Excitation wavelength: 488 nm
  • Fluorescence wavelength: red (autofluorescence)
  • Sample: Volvox
  • Observation target: Free-swimming Volvox
  • Method of imaging: Acquisition of approximately 8 volume image (stereoscopic) per second
 

C14120-20P Comparison of 3D imaging

Collaborator: Laboratory for Spatiotemporal Regulations Exploratory Research Center on Life and Living Systems (ExCELLS) / National Institute for Basic Biology (NIBB), Dr.Atsushi Taniguchi, Dr.Shigenori Nonaka

Wide field of view

C14120-20P Comparison of sensor size

Comparison of sensor size between ORCA-Flash4.0 V3 and ORCA-Lightning

 

ORCA-Lightning covers the twice large field of view compared to ORCA-Flash4.0 V3, so that it can improve throughput and efficiency of work.

Sample image 1: Test pattern

C14120-20P, Comparison of sensor size, test pattern

Imaging condition

  • Microscope: Nikon Ti2-E
  • Objective lens: CFI Plan Apo Lambda 10× / 0.45
  • F mount adapter: TI-BDTV2 +DS-F
  • Sample: Plotting objective Micrometer (Calibrated in 0.01 mm girds, 2 mm Line length)
 

Sample image 2: Dendritic spines of neuron

C14120-20P Imaging sample

Collaborator: Department of Cellular Neurobiology Graduate School of Medicine the University of Tokyo, Dr.Yutaro Kashiwagi, Dr.Shigeo Okabe

Imaging condition

  • Microscope: Nikon Ti2-E
  • Objective lens: CFI Plan Apo Lambda 100× oil / 1.45
  • F mount adapter: TI-BDTV2 +DS-F
  • Sample: GFP expressed mouse hippocampal neurons in dispersed cell culture (3 weeks culture)
  • Pixel number: 4608(H) × 2592(V)
  • Exposure time: 20 ms

Lightsheet Readout Mode [Patented]

“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.

What is Lightsheet Readout Mode?

 

Lightsheet Readout Mode

Movie gallery

Free-swimming Volvox carteri scaned by ezDSLM (Digital Scanned Light-sheet Microscope)

 

Free-swimming Paramecium bursaria scaned by ezDSLM (Digital Scanned Light-sheet Microscope)

 

GFP expressing blood cells of Oryzias latipes scaned by ezDSLM (Digital Scanned Light-sheet Microscope)

 

Collaborator: Laboratory for Spatiotemporal Regulations Exploratory Research Center on Life and Living Systems (ExCELLS) / National Institute for Basic Biology (NIBB), Dr. Atsushi Taniguchi, Dr. Shigenori Nonaka
Sample provider: National Institute for Basic Biology (NIBB), Spectrography and BioImaging Facility, Joe Sakamoto Ph. D (NBRP Medaka)

 

PC recommendation

With the introduction of the ORCA-Lightning, users are now able to stream 12 megapixel images to their computers 121 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Lightning.

Spectral response

Dimensions

 

說明 檔案大小 下載
ORCA-Lightning 產品型錄 123KB
ORCA-Lightning Technical note 123KB
型號 概述 詢價數量