若ORCA-Flash4.0 V3是高階螢光影像入門，ORCA-Fusion BT 就是螢光界的頂峰
Hamamatsu 自己研發的背照式晶片，再次將 sCMOS推向另一個世界。即使同爲背照式晶片 sCMOS，也沒辦法與 BT比較。背照式晶片最大的亮點在於其量子效率 Quantum efficiency (QE)有感提升，從82%提升至95%。讓Hamamatsu sCMOS也能踏入EM-CCD的應用領域，不論是單分子成像 (single molecule imaging)或超解析顯微鏡成像 (super resolution microscopy imaging)。ORCA-Fusion BT將不亞於 EM-CCD, 反而還提供了更高解析度，更大視野範圍 (更適用於60X水鏡/油鏡)，更高速的單分子成像及超解析顯微鏡成像。此外，Fusion BT的收光範圍也納入常用的第四通道螢光 Near-infrared (600nm-900nm)。目前大部分 CCD/CMOS在 NIR區的成像上都非常的困難，但對 Fusion BT將不是問題。
Hamamatsu BT advantages versus other BT sCMOS cameras
Exclusive industry leading BT sCMOS performance with trusted Hamamatsu quality and reliability
- Extremely low readout noise
- High QE, across a wide range of wavelengths
- CCD-like uniformity; minimal high readout noise pixels
- High dynamic range
- 3 readout speeds
- Lightsheet readout mode
- 5.3 Megapixels
- Both USB 3.0 and high speed CoaXPress interfaces
ORCA-Fusion BT advantages versus EM-CCD cameras
A practical and functional replacement for expensive EM-CCDs with uncompromising sensitivity, speed and versatility
- Resolution
- Binning versatility
- No amplification or excess noise
- High dynamic range
- Large field of view
Applications
- Wide-field fluorescence microscopy
- Time-lapse imaging
- Spinning disk confocal microscopy
- Lightsheet microscopy
- Super resolution precision localization
- Genetically encoded voltage imaging
- Optogenetics
- Single molecule tracking
- Computational imaging
- Quantum computing
Time-lapse live cell imaging by low light excitation
Sample: H9c2, Objective lens: Apo TIRF 60XC (NA1.49),
Nucleus: HCS NuclearMask Stains
Mitochondria: MitoTracker
Membrane: CellMask Plasma Membrane stains
Lightsheet Readout Mode [Patented]
“Lightsheet Readout Mode”
is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.
What is Lightsheet Readout Mode?
PC recommendations
With the introduction of the ORCA-Fusion BT, users are now able to stream 5 megapixel images to their computers 89 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Fusion BT.